| Abstract [eng] |
RNA viruses such as SARS-CoV-2 continuously evolve as changes in the genetic code occur during replication of the genome. These genetic variations may impact the properties of the virus such as transmission, severity of symptoms or sensitivity to treatments. Thus, monitoring and detection of new genomic variants is important for efficient epidemic control. Currently, the most widely used method for SARS-CoV-2 variant identification is by sequencing the whole viral RNA genome using an amplicon based system. OTDDN‘s (Oligo Tethered Dideoxy Nucleotides) technology opened the opportunity for simplified semi-targeted NGS library preparation where molecules of interest (DNA or RNA) can be captured and sequenced using only one primer. The aim of this work is to demonstrate suitability of OTDDNs in viral genomic RNA sequencing library preparation for SARS-CoV-2 RNA variant identification. |
