| Abstract [eng] |
The focus of this dissertation is on the solid surface immobilized artificial phospholipid membranes. One of the main applications of these membranes – the research of the interaction with various proteins, in other words, the immobilized phospholipid membranes should serve as biosensors. However, these biosensors have not been fully investigated due to a several challenges: 1) the technique of research – neutron reflectometry – is the best for structure‘s determination, but it is very expensive and cannot be found in every laboratory; 2) a formation of the biosensor is complicated. Thus, in this work a simpler method of analysis - surface plasmon resonance (SPR) – has been applied, and demonstrated how using the SPR method makes it possible to investigate thoroughly the composition and structure of the biosensor. It has also been adapted to a new method of preparation of the biosensors – multilayer liposomes, which don‘t require a lot of time and special equipment. In this work, a lot of attention paid to the regulation of cholesterol – one of the most important lipid in the cell membranes, because cholesterol is also responsible for some of the proteins interaction with membranes. This especially includes the pore – forming toxins, which are forming a variety of detriments in the membrane to thwart the cell life cycle, sometimes it leads to the cell death. Therefore, in this work just shows how cholesterol affects the activity of the toxin in the membranes. |
