Title Copper nanoparticle loaded electrospun patches for infected wound treatment: from development to in-vivo application /
Authors Butsyk, Anna ; Varava, Yulia ; Moskalenko, Roman ; Husak, Yevheniia ; Piddubnyi, Artem ; Denysenko, Anastasiia ; Korniienko, Valeriia ; Ramanavičiūtė, Agnė ; Banasiuk, Rafal ; Pogorielov, Maksym ; Ramanavičius, Arūnas ; Korniienko, Viktoriia
DOI 10.3390/polym16192733
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Is Part of Polymers.. Basel : Multidisciplinary Digital Publishing Institute (MDPI). 2024, vol. 16, iss. 19, art. no. 2733, p. [1-18].. eISSN 2073-4360
Keywords [eng] chitosan ; copper nanoparticles ; electrospinning ; Escherichia coli ; infected wound ; nanomedicine ; polylactic acid ; Pseudomonas aeruginosa ; Staphylococcus aureus ; wound patches
Abstract [eng] This study investigates the development and application of electrospun wound dressings based on polylactic acid (PLA) nanofibers, chitosan, and copper nanoparticles (CuNPs) for the treatment of purulent skin wounds. The materials were evaluated for their structural, antibacterial, and wound healing properties using an animal model. PLA/Ch-CuNPs demonstrated the most significant antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, surpassing the other tested materials. The integration of CuNPs into the nanofiber matrices not only enhanced the antimicrobial efficacy but also maintained the structural integrity and biocompatibility of the dressings. In vivo experiments using a rat model showed that PLA/Ch-CuNPs facilitated faster wound healing with reduced exudative and inflammatory responses compared to PLA alone or PLA-CuNPs. Histological and immunohistochemical assessments revealed that the combination of PLA, chitosan, and CuNPs mitigated the inflammatory processes and promoted tissue regeneration more effectively. However, this study identified potential toxicity related to copper ions, emphasizing the need for careful optimization of CuNP concentrations. These findings suggest that PLA/Ch-CuNPs could serve as a potent, cost-effective wound dressing with broad-spectrum antibacterial properties, addressing the challenge of antibiotic-resistant infections and enhancing wound healing outcomes.
Published Basel : Multidisciplinary Digital Publishing Institute (MDPI)
Type Journal article
Language English
Publication date 2024
CC license CC license description