| Title |
Surface plasmon resonance immunosensor for direct detection of antibodies against SARS-CoV-2 nucleocapsid protein / |
| Authors |
Lisytė, Viktorija ; Kaušaitė-Minkštimienė, Asta ; Brasiūnas, Benediktas ; Popov, Anton ; Ramanavičienė, Almira |
| DOI |
10.3390/ijms25168574 |
| Full Text |
|
| Is Part of |
International journal of molecular sciences.. Basel : MDPI. 2024, vol. 25, iss. 16, art. no. 8574, p. [1-13].. ISSN 1661-6596. eISSN 1422-0067 |
| Keywords [eng] |
antibody ; immunosensor ; SARS-CoV-2 nucleocapsid protein ; surface plasmon resonance |
| Abstract [eng] |
The strong immunogenicity of the SARS-CoV-2 nucleocapsid protein is widely recognized, and the detection of specific antibodies is critical for COVID-19 diagnostics in patients. This research proposed direct, label-free, and sensitive detection of antibodies against the SARS-CoV-2 nucleocapsid protein (anti-SCoV2-rN). Recombinant SARS-CoV-2 nucleocapsid protein (SCoV2-rN) was immobilized by carbodiimide chemistry on an SPR sensor chip coated with a self-assembled monolayer of 11-mercaptoundecanoic acid. When immobilized under optimal conditions, a SCoV2-rN surface mass concentration of 3.61 ± 0.52 ng/mm2 was achieved, maximizing the effectiveness of the immunosensor for the anti-SCoV2-rN determination. The calculated KD value of 6.49 × 10−8 ± 5.3 × 10−9 M confirmed the good affinity of the used monoclonal anti-SCoV2-rN antibodies. The linear range of the developed immunosensor was from 0.5 to 50 nM of anti-SCoV2-rN, where the limit of detection and the limit of quantification values were 0.057 and 0.19 nM, respectively. The immunosensor exhibited good reproducibility and specificity. In addition, the developed immunosensor is suitable for multiple anti-SCoV2-rN antibody detections. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2024 |
| CC license |
|
