| Title |
Synthesis and effect of 4-acetylphenylamine-based imidazole derivatives on migration and growth of 3D cultures of breast, prostate and brain cancer cells / |
| Authors |
Golcienė, Božena ; Vaickelionienė, Rita ; Endriulaitytė, Ugnė ; Mickevičius, Vytautas ; Petrikaitė, Vilma |
| DOI |
10.1038/s41598-024-76533-4 |
| Full Text |
|
| Is Part of |
Scientific reports.. Heidelberg : Nature portfolio. 2024, vol. 14, iss. 1, art. no. 28065, p. 1-16.. ISSN 2045-2322 |
| Keywords [eng] |
3D cell cultures ; alkylation ; anticancer activity ; cell migration ; imidazole |
| Abstract [eng] |
In this study, we have synthesized novel 4-acetophenone moiety-bearing functionalized imidazole derivatives containing S-, and N-ethyl substituents and evaluated their anticancer activity. Their anticancer activity was studied against human breast carcinoma (MDA-MB-231), human prostate carcinoma (PPC-1), and human glioblastoma (U-87). Compounds 4, 9, 14, and 22 were identified as the most promising anticancer agents from a series of imidazole derivatives. They showed the highest cytotoxicity by MTT assay against MDA-MB-231, PPC-1 and U-87 cell lines. Compounds 14 and 22 were most selective against PPC-1 and U-87 cell lines, and their EC50 values against these cell lines ranged from 3.1 to 47.2 µM. Most tested compounds showed lower activity against the triple-negative breast cancer MDA-MB-231 cell line. None of the imidazole derivatives possessed an inhibiting effect on the migration of PPC-1 and U-87 cells by 'wound' healing assay. In spheroid assay, the most promising were compounds 14 and 22, especially in PPC-1 3D cultures. They efficiently reduced both the size and the viability of PPC-1 spheroid cells. |
| Published |
Heidelberg : Nature portfolio |
| Type |
Journal article |
| Language |
English |
| Publication date |
2024 |
| CC license |
|
