| Abstract [eng] |
This thesis investigates the role of immunological biomarkers in non-invasively collected amniotic fluid to predict fetal inflammatory response syndrome (FIRS) and adverse outcomes in preterm neonates. A prospective cohort study was conducted at Vilnius University Hospital Santaros Klinikos, enrolling singleton pregnant women with preterm premature rupture of membranes before 34 weeks of gestation and their newborns after birth. Amniotic fluid samples were collected vaginally and analyzed for key biomarkers. The study identified specific markers – TNF-α, MMP-8, IL-6, IL-10, IL-17, DEFA3, and suPAR – as reliable predictors of FIRS, with TNF-α demonstrating the highest predictive value. Elevated levels of these biomarkers were linked to major neonatal complications, while decreased EGF levels were associated with severe respiratory issues and other adverse outcomes. These findings support a promising, non-invasive diagnostic approach for early FIRS detection and risk assessment in preterm neonates, offering the potential to enhance perinatal care and improve neonatal outcomes. |
