| Abstract [eng] |
The aim of this work was to estimate the effect of membrane permeabilizing compounds and multidrug resistance pump inhibitors on interaction of bacterial envelope with TPP+ ions. To achieve the aim we worked on antimicrobial peptides and their interaction with bacterial envelopes, on estimation of interaction of M. smegmatis envelope with TPP+ ions and equilibration of these ions across mycobacterial envelope. We have found that antimicrobial peptides pVEC and cys-pVEC have very similar effect on the envelope of gram-negative E. coli cells. They have little permeabilizing effect on the outer membrane but effectively depolarize the plasma membrane of E. coli. The peptide pVEC has depolarizing effect on both B. subtilis and M. smegmatis plasma membrane. We described interaction of M. smegmatis envelope with TPP+ ions. Polymyxin B has no permeabilizing effect on the mycobacterial cell wall, but it depolarizes the plasma membrane of M. smegmatis. Multidrug resistance pump inhibitor chlorpromazine increases the efficacy of ampicillin against M. smegmatis and has a depolarizing effect on mycobacterial plasma membrane. New potential multidrug resistance pump inhibitors have no effect on M. smegmatis sensitivity to ampicillin, however some of the compounds interacted with TPP+ efflux pumps. |
