| Abstract [eng] |
SUMMARY Comparing iron deficiency anemia and anemia of chronic disease using laboratory diagnostic indices Objective. The objective of this work is to research iron deficiency anemia (IDA) and anemia of chronic disease (ACD) laboratory diagnostic indices. Comparison anemia, inflammation and iron metabolism process reflected parameters between IDA and ACD. Material and methods. The study investigated 40 persons with IDA and 20 persons with ACD treated in the Klaipeda University Hospital. In the analyzer ARCHITECT® c 8000 tested serum iron using ferene by spectrofotomecric, ferritin and CRP concentration by immunoturbidimetric method. Blood samples were collected in EDTA and analyzed using automated hematology analyzer (Sysmex XE-2100), according to the radio - frequency/direct-current detection method, hydro dynamic focusing, flow cytometry method and SLS-hemoglobin method. Obtained the results for CBC count, including values for hemoglobin, hematocrit, RBC count and RBC indices (MCV, MCH, MCHC, RDW), WBC count and differentiation and PLT count. Was measured the erythrocyte sedimentation rate according to the standard Westergren method. All statistical analyses, including 2 – tailed t-test (unpaired) and correlation analyses were performed using STATISTICA 7 (StatSoft, Inc. 2004). Results. Comparing IDA and ACD using anemia reflect laboratory diagnostic indices hemoglobin, hematocrit, RBC count and RBC indices. Detected, that the hemoglobin concentration, MCV, MCH, MCHC indices statistically significant higher in patients with ACD and RDW statistically significantly higher in patients with IDA. Assessing of iron metabolism differences reflected indices in the sample groups. Compare the analysis of inflammatory process reflecting markers (CRP, ferritin, erythrocyte sedimentation rate, WBC count and the immature granulocyte fraction) and identify statistically higher averages for persons with ACD compare with IDA. Conclusions. Compared IDA and ACD using anemia reflect laboratory diagnostic indices detected statistically significantly differences between sample groups. Patients with IDA dominated microcytic hypochromic anemia and ACD – normocytic normochromic anemia. Assessing of iron metabolism reflected indices between IDA and ACD patients, detected statistically significantly higher values in ACD group. The analysis of inflammatory process reflecting markers identified statistically higher values in ACD compared with IDA. The assessment of inflammation process reflecting markers patients with ACD received a statistically reliable the strong correlation between the ferritin and the CRP and that show than inflammation process can influence ferritin concentration. |
