| Abstract [eng] |
The aim of this study was to determine histopathological and immunohistochemical differences of different etiologically originated immune and degenerative synovitis. To achieve this objective following tasks where raised: determine the histopathological differences between the immune and degenerative synovitis; determine immunohistochemical expression differences of TNF-α, IL-6, MMP-8 in the immune and degenerative synovitis biopsies. Histopathological and immunohistochemical analysis of synovial tissue biopsies were performed to achieve these tasks. Biopsy samples of sinovial tissue of 24 patients (11 men, 13 women) who have joint damage were used in this analysis. Of these, 12 patients were suffering from the immune synovitis and 12 patients suffering from degenerative synovitis. Performed histopathological study revealed similarities between the immune and degenerative synovitis. However, one can distinguish only a few histopathological features, which were more specific to immune synovitis that is: higher synoviocyte hyperplasia, fibroblasts proliferation is larger, lymphoid follicle formation are more frequent and increased. Immunohistochemical study revealed that both study groups (imunne and degenerative synovitis) were characterized by pronounced inflammation of synovial tissue symptoms, although the immune synovitis revealed a greater degree of inflammation than the degenerative synovitis. In both groups inflammatory cytokine TNF-α and IL-6 exhibited more strong in the blood vessel walls and synovia intima cells, and MMP-8 expression – in fibroblasts. While summarising research results it can be concluded that the microscopic biostructural studies in early stages of investigated pathologies did not reveal any undisputed immunohistochemical and histopathological features, which can be differentiate patient-specific immune and degenerative synovitis, in any stage of synovitis development, especially in the early pathological stage. However, our analysis revealed that specific symptoms are more characteristic to degenerative or immune synovitis. Thus, we found that the immune synovitis histopathologicaly differs from degenerative synovitis by - higher synoviocyte hyperplasia; increased fibroblasts proliferation; more frequent and increased lymphoid follicle formation; in immune and degenerative synovitis biopsies TNF-α, IL-6, MMP-8 immunohistochemical expressions has not revealed unambiguously patognomical differences. |
