| Abstract [eng] |
SUMMARY COMMONLY KRAS GENE MUTATION TESTING IN PATIENTS WITH METASTATIC COLORECTAL CANCER BY REAL TIME POLYMERASE CHAIN REACTION Master‘s degree final scientific research work Author of the Master‘s degree scientific research work: Elena Džervienė Head of the Master‘s degree scientific research work: prof. dr. Arvydas Laurinavičius Vilnius, 2011 Keywords: KRAS gene mutation, RT-PCR. The aim of the research work was to evaluate the commonly KRAS gene mutations in patients with metastatic colorectal cancer by real time polymerase chain reaction. The main goals of the work: 1. To evaluate incidence and diversity of commonly KRAS gene mutations. 2. To evaluate links among commonly KRAS gene mutations, patients sex, age and clinical diagnosis. 3. To evaluate the raliability of KRAS gene mutations tests. Survey methods. Commonly KRAS gene mutation testing by RT-PCR and reverse hybridization methodes. Statistical analysis made using MS Office Excel 2003 and SPSS 13.0 for Windows version statistical program. Results and conclusions. After survey, in 9 of 24 cases, KRAS gene were mutated, in 15 cases – wild type. Two types of mutation repeated more recently (3 times): p.Gly12Asp and p.Gly12Cys. More often (3 times) p.Gly12Cys mutation were found in younger patients group; p.Gly12Asp mutation (2 times) - in older ones. KRAS gene mutation relatively more often were set in womens, than in mens group. KRAS gene mutation rate were similar in both age groups (p<0,05). There were more patients with rectum 13 (54%), than colon 11 (46%) cancer diagnosis. More often mutated KRAS gene were set in patients with rectum 6 (25%), than colon 3 (13%) cancer. The similar situation was in mens group. The colon cancer in similar rate occured in both age goups. The rectum cancer more often occured in older (≥60 - 9 (37%) age than in younger one (<60 - 4 (17%)). Both localizations colorectal cancer more often were found in mens group, older than 60 years (12). In womens goup, both localizations colorectal cancer more often were found in younger than 60 years (7). Wild type KRAS gene rate were similar in both colorectal cancer localizations. P.Gly12Asp mutation more often was found in rectum cancer, p.Gly12Cys - in colon cancer. All 3 times p.Gly12Cys mutation were set especially for womens in both colorectal cancer localizations. KRAS gene mutation validation test was made sucessfully (100%) in VPC. |
