| Abstract [eng] |
Summary Electrophoretic whole-cell protein profile analysis (WCPP) – is a method providing phenotypic information. It is widely used to group bacterial strains and estimate their primal identity. In most cases reliability of this method is equal to DNA-DNA hybridization – that is the main method of genotypic information. WCPP is performed quickly and it is cheap. Therefore this method is widely used in laboratories of medical diagnostic and laboratories related to food industry. In modern world termostable enzymes are very important in industry, whereas thermophilic bacterial strains of the genus Geobacillus are producers of these enzymes. Consequently genus Geobacillus is an actual object of research. It is very important to strictly identify bacterial strains producing needed enzymes. One of the primal ways for identification of this genus could be WCPP. During this work it was shown that similarity of strains of the genus Geobacillus can be determined by WCPP. There was an 80% similarity among strains of G. stearothermophilus. These results matched with DNA-DNA hybridization of these strains. Also there were similar results of WCPP and DNA-DNA hybridization between strain 22 and typical strain of G. vulcani: 84% and 93,9% respectively. Results of WCCP denied dependence of strain G. stearothermophilus 10 DSM 13240 to this species. According to similarity dendrogram this strain should be assigned to G. vulcani because of its 97% similarity to this species. There were also WCPP results which disagreed with previous publications: G. uzenensis DSM 13551T and G. subterraneus DSM 13552T showed 90% similarity by WCPP, but only 49% by DNA-DNA hybridization. G. gargensis DSM 15378T and G. thermocatenulatus DSM 730T showed 100% similarity by WCPP, but only 43% by DNA-DNA hybridization. Identity of these strains should be revised by repeated analysis of polyphasic taxonomy. Thus, it was settled that WCPP is an appropriate method to determine relationships of species and strains of the genus Geobacillus. |
