| Abstract [eng] |
Ticks of genus Ixodes that infect livestock, deer, dogs, and a wide variety of other species including humans. Ixodes ricinus can also transmit numerous diseases including tick-borne encephalitis, Lyme diseases (Borrelia burgdorferi sensu lato infections), babesiosis (Babesia microti and Babesia divergens infections). It can also spread Anaplasma phagocytophilum bacteria. Now tick-borne infections are the most frequent human vector-borne infections in Europe, the incidence of these infections has been on rise, and new infections have emerged. In recent years, molecular detection methods based on PCR amplification of the nucleic acids of pathogens and Reverse Line blot hybridization have been showed to be effective, sensitive and specific methods for diagnosis of tick-borne diseases. In this work were used molecular detection methods for tick-borne pathogens: tick-borne encephalitis virus (TBEV), Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Babesia spp., diagnostics in 2200 ticks collected in four Lithuanian regions forests. During investigation were identified six cases of TBEV, which five positive samples were from ticks collected in Radviliškis region and one from Utenos region forest. Anaplasma phagocytophilum bacteria infections were most frequent in Lithuanian ticks, during researches were identified 37 (3 %) cases (17 (46 %) in Kėdainių region, 15 (32 %) – Klaipėdos, 5 (14 %) – Radviliškio, and 3 (8 %) – Utenos region). In these 81 % were in adult ticks and 19 % - in nymphs. During investigation of Babesia species were identified one Babesia microti in Utenos region and 2 Babesia spp. cases (Utenos, Radviliškio region forests). Borrelia burgdorferi sensu lato investigation gave no results. |
