| Abstract [eng] |
SUMMARY The prevalence of antibodies agaisnt nuclear antigens in patients in systemic connective tissue diseases The aim of this study was to assess the diagnostic value and to determine the frequency of ENA/ANA in systemic connective tissue diseases (SCTD) and to compare the association of these antibodies with following inflammatory markers ESR, CRP and rheumatoid factor (RF). The analysis involved 50 patiens with seven different diagnosis of connective tissue diseases: mixed connective tissue disease (MITD), systemic lupus eritromatosus (SLE), Sjogren syndrome, systemic sclerodermia, nondifferential connective tissue disease, dermatomiositis/ polimiositis and arthropaties. Sera from each patient was tested for ANA by indirect immunofluoresce (NIF), we assigned, that speckled pattern is appropriate for 60% and homogenous pattern is appropriate for 36% SCTD patients. The result was confirmed by immunoblot method, we estimated, that the prevalence of ENA is as follows anti-Ro-52 (56%), anti-SS-A (44%), anti-SS-B (26%), ds-DNR and nukleosomal antibodies (24%), nRNP/Sm, Scl-70 and hystonic antibodies (14%). Anti-Jo-1 (10%), anti-CENP-B (8%) anti-PCNA (4%). Antibodies to ribosomal and P- Prot antigens (2%). Antibodies to Ku antigens were not detected. Assessing the value of ENA antibodies in differentiation of SCTD, we defined that antibodies against Ro-52 antigens are the only markers detectable in all disease groups, so it could be appraised as a markers of autoimunic systemic diseases. In estimation of the relation of ANA/ENA antibodies and inflammatory markers ESR, CRP and rheumatoid factor RF, we discovered, that the highest ESR level was in association with nRNP/Sm antibodies - 60,6±38,7 mm/h, highest CRP level is in association with Scl-70 antibodies - 15,6 ± 20,8 mg/l. Highest RF level is in conjunction with SS-B antibodies 41,0±29,7 IU/L. In comparison of speckled and homogenous pattern, significant difference was not established. |
