| Abstract [eng] |
The objective of this disertation was to evaluate the role of mosquitoes (Culicidae) and biting midges (Ceratopogonidae, Culicoides) in the transmission of avian blood parasites (Haemosporida (Apicomplexa) and Trypanosomatidae (Euglenozoa)). The dissertation consists of an introduction, a literature review, research methods, results, their conclusions, a list of references, and a summary. During this research Culex pipiens mosquitoes were proven to be likely natural vectors of Plasmodium matutinum lineage pLINN1 as in mosquito salivary glands sporozoites of P. matutinum pLINN1 were microscopically detected for the first time. Molecularly P. ashfordii (pGRW02) was detected in bloodsucking insect (Ochlerotatus sticticus) for the first time. Previously, it was reported only in birds. Trypanosoma trinaperronei was found in C. pipiens mosquitoes for the first time. Hibernating C. pipiens were uninfected with haemosporidian parasites and thus can be recommended for experimental research using the natural population of wild-caught (laboratory non-reared) insects. For the first time, sporozoites of 5 genetic lineages of haemosporidian parasites were found in Culicoides kibunensis, 5 lineages in C. pictipennis, 4 lineages in C. segnis, and 1 in C. festivipennis and C. reconditus biting midges. For the first time, sporozoites of genetic lineages hCUKI1, hCULPIC02, hROFI1, hSYAT01, hSYAT13, hSYAT16 of Haemosporida spp. were found in salivary glands of Culicoides spp. |
