| Abstract [eng] |
Stereo- and regioselective synthesis of alcohols is a difficult task in organic chemistry. Therefore, the discovery of new and efficient biocatalysts followed by their investigation, application and improvement is very important for the development of target compounds. In this study, the process of dihydroxyacetone phosphate production was improved by creating the unique reactor, which eliminated hydrogen peroxide. Moreover, the stereoselectivity and substrate specificity of PQQ-dependent ADH from Pseudomonas putida HK5, and NADPH-dependent short chain dehydrogenase/reductase TpdE from Rhodococcus jostii TMP1 were investigated. The potential of these enzymes as efficient biocatalysts for the production of chiral alcohols was demonstrated. In addition, free and alginate gel-entrapped cells of Burkholderia sp. MAK1 were studied as a whole cell biocatalyst for the synthesis of hydroxylated N-heterocyclic compounds. In total, 73 out of 110 tested compounds were transformed by Burkholderia sp. MAK1. The analysis of the products revealed that the hydroxylation was regioselective. Thus, the cells of Burkholderia sp. MAK1 can be applicable for the regioselective synthesis of pyridinols. |
