| Abstract [eng] |
A biosensor is an analytical device that inverts a biochemical reaction process into a measurable signal using transducer. Mainly these devices are used for detecting various substances like pollutants, metabolites, microbial load. The main domain of usage is medicine and food industries. Usually biosensors consist of a biological sensing element - enzyme and a transducer for detecting the analyte concentration. Unfortunately experiments in laboratory are expensive, for this reason creating computer-aided models of such systems is very attractive. The main purpose of such modelling is to study the peculiarities of biosensors, developing their attributes according to environmental settings. A sensitivity of biosensor expressed by linear range of the calibration curve was chosen as the main characterics for developing. The mathematical models of layered amperometric biosensors were analyzed. Solving a biosensor model numerical methods, a finite difference scheme is used. A software tool for computational modelling for amperometric biosensors in flow injection systems, the multilayered biosensors as well as optimization-based evaluation of concentrations in modelling the biosensor-aided measurement was created. In thesis the solutions were found considering dimensionless models, and generalized results of the research are presented. The results can be used practically and for further development as well. |
