| Abstract [eng] |
Artificial phospholipid bilayer membranes were designed and investigated in biochemical studies involving protein-lipid interaction. Several different models of phospholipid bilayers on solid supports were designed that mimics the structure of plasma membrane. Two of the most studied membrane models are hybrid bilayer lipid membrane (hBLM) and tethered bilayer lipid membrane (tBLM). In this work, thin film cadmium tin oxide and fluorine doped tin oxide (FTO) surfaces were used as alternatives for typically used gold surfaces for the formation of bilayers. The formation of self-assembled monolayer (SAM) on oxide surfaces was achieved via silane chemistry that ensured strong covalent bond formation. The biological relevance of hBLMs on FTO were tested with different bacterial or venom toxins: phospholipase A2 (PLA2), melittin (Mel), α-hemolysin (αHL) and vaginolysin (VLY). hBLMs were disrupted by melittin and PLA2 in concentration dependant manner, however pore forming toxins αHL and VLY could not penetrate the membranes. Therefore, new silane compound were synthesised for SAM formation that ensured thin water reservoir formation between solid surface and bilayer during vesicles fusion. The existence of water reservoir allows functional reconstitution of pore forming toxins into tBLMs on oxide surfaces and open up opportunities for development of regenerable biosensors for pore forming toxins detection. |
