| Title | Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2) / |
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| Authors | Petruškevičiūtė, Aistė ; Šimuliūnaitė, Ugnė ; Polanco, Catalina M ; Rojas, Barbara ; Kuras, Simonas ; Valatkaitė-Rakštienė, Beatričė ; Norvilas, Rimvydas ; Vijaya, Akshay Kumar ; Muñoz, Patricia ; Neniškytė, Urtė ; Jakubauskas, Artūras ; Burokas, Aurelijus ; Nalvarte, Ivan ; Inzunza, Jose ; Naumovas, Daniel ; Stoškus, Mindaugas ; Griškevičius, Laimonas ; Baltriukienė, Daiva ; Arias, Jonathan |
| DOI | 10.1016/j.scr.2024.103628 |
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| Is Part of | Stem cell research.. Amsterdam : Elsevier B.V.. 2025, vol. 82, art. no. 103628, p. [1-6].. ISSN 1873-5061. eISSN 1876-7753 |
| Keywords [eng] | Cas9 ; CRISPR ; genetically encoded reporter ; iPS cells ; MARINA |
| Abstract [eng] | Fluorescent protein-based Genetically Encoded Voltage Indicators (GEVI) offer a remarkable system for high-throughput screening of membrane potential phenotypes. The GEVI MARINA is a derivative from ArcLight, which conversely to ArcLight increases its fluorescence intensity alongside depolarization. Here we created knock-in reporter human iPS cell lines carrying the MARINA reporter using SpCas9 programmable nuclease and characterize a heterozygous clone. |
| Published | Amsterdam : Elsevier B.V |
| Type | Journal article |
| Language | English |
| Publication date | 2025 |
| CC license |
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