| Abstract [eng] |
Pyridine and its derivatives are widespread environmental pollutants originating from natural and anthropogenic sources. While microbial degradation of pyridine and pyridinols is recognized, the underlying enzymatic mechanisms remain incompletely understood. This thesis explores novel catabolic pathways and enzymes involved in the microbial metabolism of N-heterocyclic aromatic compounds. Four newly identified pathways provide key insights into bacterial adaptation to pyridine-based compounds, expanding our understanding of microbial detoxification. The identified biocatalysts highlight the evolutionary ingenuity of bacteria in responding to chemical diversity in their environments. Beyond fundamental research, this work highlights the biotechnological potential of microbial enzymatic diversity. The novel enzymes show promises and prospects for bioremediation, green chemistry, and synthetic biology. By elucidating novel metabolic routes, it contributes to the development of sustainable industrial processes and innovative environmental remediation strategies, bridging basic science with applied solutions for ecological challenges. |
