| Abstract [eng] |
Endothelial microvesicules (EMV) are complex vesicular structures shed from activated or apoptotic endothelial cells and carry inside a variety of proteins and genetic material that may influence the cell processes. They play a role in coagulation, inflammation, endothelial function, and angiogenesis and thus may be marker for early vascular diseases. In the present study, the presence of endothelial microvesicules in healthy male plasma were investigated using flow cytometry and compared to endothelial microvesicules shed from endothelial cells effected by cyclophilin A in vitro. Healthy subjects were divided into two groups according to cholesterol concentration. Results have shown that in lower cholesterol concentration group, high sistolic blood pressure positively correlated with EMV CD105+. Compared with other group, where cholesterol concentration was higher, EMV CD105+ had negative correlation with concentration of low density lipoproteins. Though second group have shown positive correlation between systolic blood pressure and EMV CD62e+, CD144+, CD42a-. EMP CD62e+, CD144+ CD42a- also were elevated in vitro influenced by cyclophilin A at concentration of 10ng/mL. EMV increase was not observed at higher cyclophilin A concentrations. We believe apoptotic effect of cyclophilin A on endothelial cells could be blamed. These in vivo and in vitro observations indicate that endotelium activation level may be observed by identifying and counting EMV Future research is needed to better evaluate EMV as diagnostic marker for early vascular disease. |
