| Abstract [eng] |
Alcohol use disorders (AUD) – a tremendous issue in modern society, which affects nearly all the countries in the modern world. Lithuania – a leading country, according to pure alcohol consumption, in the world. An abusive alcohol use directly and indirectly affects persons’ physical, psychological and social health. It even reaches a global scale – where its’ effects are seen in country’s economy, demography, or crime statistics. Therefore, it is important to research the primary cause of AUD. The aim of the thesis – to find new and check known genetic factors which are associated with alcohol use dynamics in Lithuanian population. To archive the goal, molecular genetic methods where used, such as, SNP–CGH or Sanger sequencing. Sample consisted of 300 adult Lithuanians, who participated in “Lithuanian population genetic diversity and structure changes, related to the evolution of the most prevalent diseases” (LITGEN) project and answered to questionnaire’s question – “Do you drink alcoholic beverages?”. SNP–CGH generated data analysis showed interpopulational and intrapopulational differences (rs8188000 and rs1799971), between case and control groups, which can affect the dynamics of alcohol use. Sanger sequencing generated data (N = 144) analysis failed to show any association with alcohol use dynamics and used genetic markers (rs1229984, rs698, rs2238151), but it would be wrong to reject their effect. To further analyze the effects of environmental factors logistic regression models were used. Fitted model showed that males (OR = 3.671 (CI 95% 3.840–39.273), p = 2,36-05) and smokers (OR = 3,668 (CI 95% 3.405–39.162), p = 8,62-05) are more likely to frequently use alcoholic beverages. Furthermore, it was observed, that people with secondary education has lower probability to frequently use alcohol beverages (OR = 0.131 (CI 95% 0.022–0.783), p = 0.0259). All the obtained results should be replicated in the study with larger sample size and more detailed questionnaire, which would help to determine more specific case and control groups. |
