| Abstract [eng] |
The aim is to assess the association with AB0 blood group system of individuals suffering from cancer, compare it with the long-lived individuals and coronary artery disease patient groups, classify research groups as per cluster analysis and investigate polymorphism of vitamin D binding protein. Tasks are:1)To perform cluster analysis for long-lived individuals and patients suffering from urinary system cancer, coronary artery disease according to AB0 blood group system; 2)To assess AB0 blood group system frequency of urogenital system and long-lived individuals; 3)To measure AB0 blood group system association with long-lived individuals and patients suffering from cancer; 4)To analyze the frequency of the vitamin D binding protein polymorphism in long-lived individuals group. Methods and material: serological analysis of AB0 blood group system, DNA purification using silica-based columns, real time PCR method, cluster analysis. The test groups: healthy blood donors (n=595); prostate (n=2200), bladder (n=1530) and renal (n=2650) cancer patients; patients with atherosclerosis (n=464); group of long-lived individuals (n=154). Research results. The population of Lithuanian women, who are suffering from renal and bladder cancer, have significantly lower blood group 0 frequency than the blood donors. Blood group A is statistically significantly lower among kidney and blander cancer patients groups than between healthy Lithuanian population. Blood type B is more often found in urogenital system cancer group compared to blood donors and long-lived individuals groups. Blood type 0 is a risk reduction factor for bladder cancer in population of ≥60 year old Lithuanian women. Studies have showed that blood type A is associated with lower risk of bladder and renal cancer, and blood group B with higher risk of urinary system cancer in Lithuanian population. Results show that Lithuanian women with blood type A have higher life expectancy, than women whose blood type is B. The cluster analysis revealed that patients suffering from urinary system cancer formed separate large cluster from blood donors, long-lived individuals and coronary artery disease groups. Distribution frequency of rs4588 and rs7041 polymorphisms did not correspond to Hardy-Weinberg equilibrium (p < 0.05). Rs4588 polymorphism of vitamin D binding protein analysis showed that the overall long-lived group were generally determined T allele (0.44), GT (0.38) and TT (0.24) genotype; as well as genotyping analysis of rs7041 polymorphism showed frequency of A allele, AC and AA genotypes, respectively 0.44, 0,38 and 0.24. Investigation of 16 long-lived individuals showed, that the most frequent vitamin D binding phenotypes are Gc1s-1s and Gc1s-2. Findings:1)The clustering analysis revealed that patients suffering from urinary system (prostate, renal and bladder) cancer formed separate large cluster from blood donors, long-lived individuals and coronary artery disease groups; 2)AB0 blood group system frequency distribution showed that blood type A is rarely found in patients group suffering from urogenital system cancer, and blood type B – often, compared to healthy population. In long-lived population, most frequent blood types are 0 and A, and less often are B and AB compared to healthy blood donors; 3)It is found, that blood group A is associated with reduced risk factor for bladder and renal cancer in healthy population and blood group B is associated with increased risk of cancer. Blood type A is also associated with increased life expectancy among the population of Lithuanian women, while blood type B – with decreased; 4)A study of 15 long lived individuals showed rs4588 and rs7041 polymorphisms of vitamin D binding protein T and A alleles. The examined long-lived individuals mainly had Gc1s-1s and Gc1s-2 phenotype. The increased Gc2-2 phenotypes frequency trend is consistent with previously published data for long-lived Lithuanian population. |
