| Abstract [eng] |
The goal of tissue engineering is to restore the function of damaged tissues and organs using biomimetic materials. A key component in this process is the extracellular matrix, whose mechanical and structural properties play a crucial role in regulating cell behaviour, regeneration, and differentiation. The development and use of materials that mimic extracellular matrix is an important part of the tissue engineering process. This thesis explores the formation and modification of collagen-based biomimetic hydrogels and evaluates their application in organotypic cell cultures. Micro- and nano-fabricated hydrogel surfaces were developed and tested with human skin fibroblasts, and an in vitro corneal model was engineered for material permeability testing. The hydrogels were also assessed for their suitability in cardiomyocyte differentiation and in supporting neural cells and cerebellar organoid formation. Furthermore, novel peptide hydrogel modifications were designed by incorporating bioactive sequences into collagen-mimicking peptides. The findings of this thesis contribute to the advancement of biomimetic platforms for complex tissue modelling in vitro. |
