| Abstract [eng] |
The relevance of the problem and the aim of the work. Biocompatibility is an essential property for materials used in root canal treatment. The aim of this research was to evaluate influence of flowable hydraulic tricalcium silicate-based cements in vitro on human periodontal ligament fibroblasts cytotoxicity and morphological changes. Materials and methods. Tested groups: positive (IRM), negative (growth medium) and ProRoot MTA control groups, Totalfill BC Sealer and BioRoot Flow. Freshly mixed cements were incubated for 48 hours, eluates were collected, pH values measured, and solutions of 100%, 50%, and 25% concentrations were prepared. Cell morphology was observed under a light microscope and analyzed using ImageJ software. MTT cytotoxicity assay was performed after 24 hours using a spectrophotometer. Measurements taken in quadruplicates. Data were analyzed using ANOVA with GraphPad Prism software. Results. Measured pH values: growth medium – 8,36; IRM – 6,38; ProRoot – 9,51; Totalfill BC – 9,26; BioRoot Flow – 9,35. After 24 hours, the 100% concentration ProRoot and BioRoot eluates significantly reduced cell viability. The 50% and 25% concentration TotalFill and BioRoot eluates increased cell viability, at the same time, maintaining lower cytotoxicity compared to ProRoot. Eluates of all cement groups resulted in a greater cell width compared to the negative control group, and cells affected by TotalFill were significantly wider than those in the ProRoot group. TotalFill and ProRoot led to a higher cell count. No differences in cell length or the aspect ratio were found. Conclusions. The freshly mixed TotalFill BC Sealer and BioRoot Flow eluates demonstrated the best biocompatibility properties. The viability and morphology of periodontal ligament fibroblasts depended on the type of tested cement and the concentration of the eluates. |
