| Abstract [eng] |
Author: Lorenz Kauth Title: Morphological Changes of Kidney in Type 1 Diabetes Mellitus Influenced by Psychological Stress. Aim: The aim of this study was to investigate how psychological stress influences the morphological changes in the kidneys of individuals with Type 1 Diabetes mellitus. Objectives: 1) To conduct a comprehensive literature analysis to identify existing research on the relationship between psychological stress, Type 1 Diabetes mellitus, and kidney morphology. 2) To examine the specific morphological changes in the kidneys of individuals with Type 1 Diabetes mellitus and compare with control. 3) To assess the impact of psychological stress on renal morphological changes among experimental groups. Research Methodology: The research included 35 mature healthy female Wistar rats, randomly divided into 4 groups: Control (n=9), Stress (n=10), DM (n=8), and the DM+Stress (n=8). Each group underwent different experimental conditions for 28 days. Diabetes was induced using streptozotocin, while chronic stress was simulated by daily restraint stress. Tissue samples were analysed for the size of renal corpuscles, Bowman’s capsule size, and the total number of renal corpuscles using QuPath 04.4 software. The data was statistically analysed using R Studio, with significance levels set at p < 0.05. Results: The results indicated that the size of renal corpuscles varied across the four groups. The stress group exhibited the largest renal corpuscles, with p < 0.05, followed by the Control group, while the DM (p > 0.05) group and the DM+Stress group (p < 0.05) had the smallest renal corpuscles. These findings suggest that stress alone may cause hypertrophy, whereas the combination of diabetes and stress might result in reduced size of renal corpuscles, indicating a possible compensatory response by the kidneys to the combined stressors. The analysis of Bowman’s capsule size showed that DM+Stress group resulted in the largest capsule, p < 0.05. The stress group showed the smallest Bowman’s capsule size, p < 0.05. These results suggest that chronic stress by itself may cause the capsule to shrink, but when stress is combined with diabetes, it seems to make the capsule enlarge even more, possibly because stress speeds up the process. In terms of the number of renal corpuscles, no statistical significance was observed between Control, the DM and DM+Stress groups. The stress group had the lowest number of renal corpuscles, p < 0.05, suggesting that stress alone can lead to a reduction in the number of renal corpuscles. Conclusions: The research findings suggest that both diabetes and psychological stress independently impact kidney morphology, even when the combination of the two does not always lead to more pronounced changes. While chronic stress significantly increases the size of renal corpuscles, it reduces the size of the Bowman’s capsule and the total number of renal corpuscles. DM and stress only appeared to increase the Bowman’s capsule size and decrease the size of the renal corpuscles. This supports the idea that both stress and DM impair the morphological effects of diabetes on the kidneys. |
