| Abstract [eng] |
The COVID-19 pandemic caused by the SARS-COV-2 virus has ended and many people have been vaccinated against this insidious disease, but mortality rates continue to rise. Therefore, it is still important to conduct research and analysis related to the SARS-COV-2 virus. The aim of this work was to develop a SPR immunosensor for the detection of specific antibodies against SARS-COV2-RBD protein using different detection formats. The applicability of SPR immunosensors of direct and two types of indirect detection formats for analyte detection was demonstrated. In the case of the direct detection format, the linear dependence range of analyte detection was from 0.5 to 50 nmol L-1, the calculated detection and quantification limits were 0.17 and 0.52 nmol L-1, respectively. In the case of indirect detection, where secondary anti-IgG antibodies were used for analyte detection, the linear dependence range was from 0.1 to 20 nmol L-1. The calculated detection and quantification limits were 2.1 times lower than in the case of the direct detection format immunosensor. In another case, an indirect immunosensor, where synthesized AuND-anti-IgG-HRP conjugates were used for analyte detection, the linear dependence range was from 0.05 to 5 nmol L-1. The calculated detection and quantification limits were 4.3 times lower than in the direct detection format. The suitability of the indirect immunosensor, with secondary antibodies for analyte detection, for analyte detection in real samples was also confirmed. |
