| Abstract [eng] |
Long-distance space missions will become a reality in the future, but the negative effects of space conditions on living organisms are obvious but not yet fully understood. It is not known how this will affect astronauts' health in the long term. The ends of eukaryotic cell chromosomes are protected from degradation by telomeres, which are affected when cells are exposed to various en vironmental stresses. Studies show that after short-term space missions, astronauts have shorter telomeres, and shorter telomeres are associated with cell aging and longevity. This work investigates the effect of microgravity on the death and resistance to antifungal agents of S. cerevisiae strains with different telomere lengths by comparing cells grown in normal gravity and simulated micro gravity. The study evaluated chemical factors such as resistance to antifungal compounds - clotrima zole and nystatin. Microgravity was simulated using a rotary cell culture system, resistance to chem ical compounds was assessed by determining MIC and evaluating the results of the clonogenic test, and cell death analysis was assessed by determining ROS formation, caspase activation and phos phatidylserine localization. Based on the results obtained, it can be concluded that the effect of MG, depending on the strain and the chosen method, can lead to increased or decreased resistance to chemical compounds. When evaluating the results of the MIC test, the effect of MG led to a decrease in the resistance of DLY 1479 and DLY 641 strains to nystatin and clotrimazole. The results of the clonogenic test show that the effect of MG led to increased resistance to antifungal compounds. A decrease in ROS for mation was observed in cells of all strains grown in the MG environment, and caspase activation was also observed in cells of the DLY 641 strain. The effect of nystatin led to an increase in the DLY 641 Annexin+ / PI- phenotype and a decrease in the number of cells with the DLY 1479 and DLY 641 Annexin+ / PI+ phenotype under microgravity conditions. |
