| Abstract [eng] |
Study of females genital tract microflora diversity SUMMARY Study of female genital tract microflora diversity Authors of Master’s degree scientific research work: Justina Vertelytė Head of Master’s degree scientific research work: dr Silvija Kiverytė Vilnius, 2016 The aim of research work was to investigate and analyze the composition of the microflora of the female genital tract using the methods of microbiological smear, vaginal wet mount and PCR. The objectives of the work were to evaluate the species of microorganisms in microbiological smears from the cervix by their abundance and by identifying the members of genital tract microflora in normal and pathological cases. Materials and methods. The study was carried out in 2015 – 2016 in the Microbiology laboratory of the Santariškės hospital Laboratory medicine center. Vaginal samples from vaginal wet mounts and cervical swabs for microbiological smears and identification of Atopobium vaginae were studied. Microorganisms from female genital tracts were cultivated in the microbiology laboratory using microbiological smear analysis methods. MALDI-TOF MS was used to identify fungi and bacteria and real-time PCR was used to identify Atopobium vaginae. Results and conclusions. We have analyzed 100 cervical samples, with the subjects being 21 to 65 years old. Normal vaginal microflora was found in 56 cases and there were 41 pathological cases. Using microbiological and molecular methods, we found 29 different species of microorganisms in gynecological smears with 2 species of fungi and 27 species of bacteria. 14 patients were diagnosed with Candida spp. fungi and 4 patients were diagnosed with Gardnerella vaginalis. Most common in the pathological cases were the aerobic vaginitis-causing bacterial species of Streptococcus agalactiae (n=20), Enterococcus faecalis (n=17) and Esherichia coli (n=13). 46% of the subjects were diagnosed with Atopobium vaginae with it being more common among younger women (21 – 39 years old (n=35)) compared to older ones (n=10). A relation between microorganisms and vaginitis was found. There was statistical significance between Candida spp. and candidosis in vaginal wet mounts (p=0,01) and between Atopobium vaginae and bacterial vaginosis in vaginal wet mounts (p=0,0001). No relations of statistical significance between Gardnerella vaginalis and bacterial vaginosis or between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis were found. Keywords: Streptococcus agalactiae; Atopobium vaginae; Gardnerella vaginalis; bacterial vaginosis; vaginal discharge; cervix; swab; smear; microbiological culture; PCR; MALDI-TOF MS. |
