| Authors |
Naimovičius, Lukas ; Miroshnichenko, Mila ; Opar, Ekin ; Hölzel, Helen ; Morikawa, Masa-Aki ; Kimizuka, Nobuo ; Dapkevičius, Manvydas ; Lekavičius, Justas ; Radiunas, Edvinas ; Kazlauskas, Karolis ; Cilleros-Mañé, Víctor ; Riefolo, Fabio ; Matera, Carlo ; Harmandar, Kevser ; Taniguchi, Masahiko ; Dumoulin, Fabienne ; Lindsey, Jonathan S ; Bharmoria, Pankaj ; Gorostiza, Pau ; Moth-Poulsen, Kasper |
| Abstract [eng] |
Red, far red, or near-infrared photoswitchable drugs offer immense photo-pharmacological advantages due to the higher light penetration through the skin. Such photoactivation is achieved using processes such as two- and three-photon absorption, excited-state absorption, and triplet-triplet annihilation upconversion, which require higher photon fluences (W to kW cm−2) than the resilience constraints of skin (200 mW cm−2). Herein, a generalized approach of cis-to-trans photoisomerization of azobenzenes is demonstrated via triplet sensitization with NIR-I illumination (850 nm) of a new Zn-octa-substituted phthalocyanine photosensitizer, in aqueous medium at 2.62 mW cm−2. The approach is applied to control the heart rate of a frog tadpole via cis-to-trans photoisomerization of an azobenzene-functionalized muscarinic acetylcholine receptor M2 agonist in the phototherapeutic window (730 nm excitation: 42 mW cm−2). This advance highlights a powerful photo-pharmacological strategy for modulation of in vivo activity at 2-4 orders of magnitude lower photon fluences of NIR light compared to established methods. |
