| Abstract [eng] |
The thesis presents a study of the cyclic Urotensin II peptide (U-II) using Raman and SERS spectroscopy methods. The Urotensin II molecule is known for its vasoconstrictor properties and is also of interest for its functions as a neurotransmitter and neuromodulator. Elevated concentrations of this peptide in the context of kidney, liver, and heart diseases make it a promising research target for drug development. Since the discovery of this molecule, numerous studies have been conducted on its biological effects and its role in diseases. However, critically few studies have investigated the molecule from a purely chemical perspective. Investigating the molecule using Raman and SERS spectroscopic methods will allow to determine its “fingerprint” spectra, understand its intra- and intermolecular interaction mechanisms, and, in the future, facilitate the creation of synthetic analogs with tailored properties. In this work, the characteristic Raman spectrum of the U-II peptide in its native state was obtained for the first time. By applying SERS and EC-SERS methods, a mechanism of U-II adsorption on various metal surfaces was proposed, and the influence of factors such as the anion and metal type on the adsorption process was investigated. This knowledge suggests a 'molecule-receptor' interaction pattern, which could facilitate the creation of synthetic analogs with similar properties. Raman and SERS spectroscopy studies have made it possible to study the Urotensin II molecule in low concentrations and in situ conditions without significant preliminary preparation. |
