Title Soil genotoxicity biomonitoring in recultivated factory area using the cytogenetic and molecular assays in two plant test-systems /
Authors Stapulionytė, Asta ; Bondzinskaitė, Skaistė ; Stravinskaitė, Monika ; Šiukšta, Raimondas ; Taraškevičius, Ričardas ; Čėsnienė, Tatjana
DOI 10.15544/RD.2017.025
eISBN 9786094491283
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Is Part of Rural development 2017: bioeconomy challenges : proceedings of the 8th international scientific conference Rural Development 2017.. Akademija : Aleksandras Stulginskis University, 2017. p. 119-127.. ISSN 1822-3230. eISSN 2345-0916. eISBN 9786094491283
Keywords [eng] Allium ; cytogenetic markers ; ISSR ; PHEs ; RAPD ; soil genotoxicity ; Tradescantia
Abstract [eng] Soil pollution with industrial leftovers is of real danger to living organisms since harmful effects can arise after exposure to the contaminants in the soil. In our study, we applied a plant bioassay battery to monitor soil genotoxicity after short-term exposure to the soil. The soil was collected in 3 rounds: at the central part of the brownfield before (S-I) and after (S-III) topsoil removal, and at the brownfield periphery (S-II). The permissible value of the total contamination index is <16 and the corresponding values were 780 in S-I, 69 in S-II and 133 in S-III soil showing that whole brownfield territory is extremely polluted with heavy metals. Cytogenetic markers were recorded in Allium and Tradescantia test-systems and two types of molecular markers, RAPD and ISSR, were analysed in Allium. Our results revealed that the most polluted soil sample has induced an alarming increase of apoptotic cells in onion roots. Chromosome aberration and micronuclei frequency in Allium decreased inconsistently along with the pollution reduction in the soil. Increased frequencies of all cytogenetic markers were revealed in Tradescantia cuttings after exposure to the S-I soil extracts. Cluster analysis of Allium RAPD and ISSR markers showed that the most polluted soil samples induced genetic changes in onions different from those induced by the least polluted soil. Both plant test-systems in this study confirm that soil from the brownfield is harmful to plants and is potentially hazardous to humans.
Published Akademija : Aleksandras Stulginskis University, 2017
Type Conference paper
Language English
Publication date 2017