| Title |
Indium tin oxide-based voltammetric biosensor for the detection of antibodies against the SARS-CoV-2 virus spike protein |
| Authors |
Žvirzdinė, Greta ; Drobysh, Maryia ; Ramanavičienė, Almira ; Ratautaitė, Vilma ; Žukauskas, Šarūnas ; Stančiauskaitė, Miglė ; Plikusienė, Ieva ; Ramanavičius, Arūnas |
| DOI |
10.3390/s25216737 |
| Full Text |
|
| Is Part of |
Sensors.. Basel : MDPI. 2025, vol. 25, iss. 21, art. no. 6737, p. [1-11].. eISSN 1424-8220 |
| Keywords [eng] |
affinity interaction ; antigen–antibody binding ; ellipsometry ; immune complex formation ; indium tin oxide (ITO) electrode ; recombinant spike protein ; SARS-CoV-2 virus ; square wave voltammetry (SWV) |
| Abstract [eng] |
This study aims to propose a plausible application of a novel electrochemical biosensing system for detecting antibodies against SARS-CoV-2 (anti-rS) in serum samples. The uniqueness of this study lies in the biosensor utilizing recombinant spike glycoprotein (SCoV2-rS) immobilized on an indium tin oxide (ITO) electrode modified with (3-aminopropyl)triethoxysilane (APTES). The electrochemical performance was evaluated using square wave voltammetry (SWV), demonstrating a linear relationship between the current density and anti-rS concentration. The limit of detection (LOD) was found to be 113 ng/mL (0.75 nM), and the limit of quantitation (LOQ) was equal to 338 ng/mL (2.25 nM). The reported electrochemical biosensor offers a straightforward and efficient method for evaluating the immune status of individuals who have recovered from COVID-19 and been vaccinated against this virus without the need for any redox probe. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2025 |
| CC license |
|
