| Title |
Phytochemical profiling of Ferula varia extract and its antibiofilm activity against Streptococcus mutans |
| Authors |
Smagulov, Marlen K ; Levaya, Yana K ; Badekova, Karakoz Zh ; Ivasenko, Svetlana A ; Atazhanova, Gayane A ; Gabė, Vika ; Ishmuratova, Margarita Yu ; Kačergius, Tomas |
| DOI |
10.3390/molecules30214178 |
| Full Text |
|
| Is Part of |
Molecules.. Basel : MDPI. 2025, vol. 30, iss. 21, art. no. 4178, p. [1-16].. eISSN 1420-3049 |
| Keywords [eng] |
antibacterial activity ; biofilm inhibition ; Ferula varia ; phytochemicals ; Streptococcus mutans |
| Abstract [eng] |
Dental caries is a major global health concern, with Streptococcus mutans playing a key role in biofilm formation and acid production, which lead to enamel demineralization. Natural products, particularly plant-derived extracts, offer promising alternatives to conventional antibacterial agents. This study aimed to analyze the chemical composition of Ferula varia 70% ethanol extract (FVE) and evaluate its potential to inhibit biofilm formation by S. mutans. The aerial parts of F. varia were extracted with 70% ethanol and analyzed using LC-UV-ESI-MS/MS to determine the chemical profile. The anti-biofilm activity of FVE was evaluated using a crystal violet assay against S. mutans. Phytochemical analysis identified 14 compounds, including major phenolic acids (e.g., chlorogenic acid, gallic acid) and flavonoids (e.g., isoquercitrin, isorhamnetin-3-O-glucoside). FVE exhibited significant, dose-dependent inhibition of S. mutans biofilm formation. Importantly, the FVE concentration of 5 mg/mL inhibited S. mutans biofilm development by 100%. The potent antibiofilm activity of FVE against S. mutans is likely due to the synergistic action of its rich content of phenolic acids and flavonoids, which possess known anti-virulence properties. These findings support the potential use of FVE as a natural ingredient in oral hygiene products to prevent dental plaque and caries. |
| Published |
Basel : MDPI |
| Type |
Journal article |
| Language |
English |
| Publication date |
2025 |
| CC license |
|
