| Title |
Optimization of polyomavirus VP1 protein biosynthesis in S. cerevisiae cells and application of recombinant virus-like particles for serology / |
| Translation of Title |
Poliomos virusų VP1 baltymų biosintezės ypatybių S. cerevisiae ląstelėse tyrimas bei panaudojimas serologiniams tyrimams” antotacija. |
| Authors |
Norkienė, Milda |
| Full Text |
|
| Pages |
50 |
| Keywords [eng] |
Polyomaviruses, Chaperones ; Flocculation ; Yeast. |
| Abstract [eng] |
In this study, a targeted genetic approach to optimize PyV VP1 proteins synthesis in S. cerevisiae cells was performed. The requirement for molecular chaperones during biosynthesis of PyV capsid protein VP1 was evaluated, the reasons of yeast flocculation caused by expression of recombinant VP1 protein derived from some PyV was investigated and the impact of NLS in WU VP1 N-termini for VLPs formation was analysed. The yeast expression system was successfully utilized for high-throughput production of recombinant VP1-derived VLPs from 11 newly identified human PyVs for the first time. Human PyV VP1-derived VLPs were generated from the second of two potential translation initiation sites in the VP1-encoding open reading frame. Recombinant VLPs produced in yeast originated from different HPyVs demonstrated distinct hemagglutinating activities (in hemagglutinating assay) and may be useful in virus diagnostics. All purified HPyV VP1 VLPs were used to develop indirect enzyme immunoassay for detection of HPyVs specific antibodies in human sera samples. Human PyVs are part of the normal microbial flora and the infection of these viruses in their host is thought to be asymptomatic but, when host immunity is compromised the infection can cause acute systemic disease or tumor induction. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Summaries of doctoral thesis |
| Language |
English |
| Publication date |
2018 |
