| Title |
Phoretic interactions and oscillationsin active suspensions of growing Escherichia coli / |
| Authors |
Šimkus, Remigijus ; Meškienė, Rita ; Aučynaitė, Agota ; Ledas, Žilvinas ; Baronas, Romas ; Meškys, Rolandas |
| DOI |
10.1098/rsos.180008 |
| Full Text |
|
| Is Part of |
Royal Society Open Science.. London : The Royal Society. 2018, Vol. 5, iss. 5, art. no 180008, p. 1-16.. eISSN 2054-5703 |
| Keywords [eng] |
bacterialgrowth ; bacterialchemotaxis ; biochemicaloscillations ; Janusparticles ; self-phoresis |
| Abstract [eng] |
Bioluminescence imaging experiments were carried out to characterize spatio-temporal patterns of bacterial selforganization in active suspensions (cultures) of bioluminescent Escherichia coli and its mutants. An analysis of the effects of mutations shows that spatio-temporal patterns formed in standard microtitre plates are not related to the chemotaxis system of bacteria. In fact, these patterns are strongly dependent on the properties of mutants that characterize them as self-phoretic (non-flagellar) swimmers. In particular, the observed patterns are essentially dependent on the efficiency of proton translocation across membranes and the smoothness of the cell surface. These characteristics can be associated, respectively, with the surface activity and the phoretic mobility of a colloidal swimmer. An analysis of the experimental data together with mathematical modelling of pattern formation suggests the following: (1) pattern-forming processes can be described by Keller–Segel-type models of chemotaxis with logistic cell kinetics; (2) active cells can be seen as biochemical oscillators that exhibit phoretic drift and alignment; and (3) the spatio-temporalpatternsinasuspensionofgrowingE.coliform due to phoretic interactions between oscillating cells of high metabolic activity. |
| Published |
London : The Royal Society |
| Type |
Journal article |
| Language |
English |
| Publication date |
2018 |
