| Abstract [eng] |
CRISPR/Cas9 is a powerful tool, that is being used in the most recent research. Interest in this technology is rising every year. The main aim of this study was to investigate restriction endonuclease BcnI DNR sequence snipping and recognition mechanisms, to analyze ongoing processes and to understand the principles of operation of mechanisms mentioned. The work of this research is focused on short pallindromic repeats in DNA and restriction endonuclease BcnI. Restriction endonuclease BcnI DNA sequence snipping and recognition mechanisms were analized with the help of quantum chemistry methods. Modeling results helped to get answers for most of the questions, analize hipothesis, that were presented. Hydrogen bonding network is an additional factor, that is involved in the DNA sequence snipping mechanism. The obtained simulation results allow to conclude that the hydrogen bonding network reduces the stability and strength of the molecular system, which allows the restriction endonuclease BcnI to cut the DNA sequence. |
