| Abstract [eng] |
The Value of the Cintec Plus Immunocytochemistry Test for the Diagnosis of Cervical Pathology, Comparing with the Results of Thinprep PAP and Human Papilloma Virus DNA Tests SUMMARY Clinical value of the Cintec Plus Immunocytochemistry test for the diganosis of cervical pathology, comparing with the results of Thinprep PAP and human papiloma virus DNA tests Infection by the human papillomavirus (HPV) is the most important risk factor for cervical cancer. HPV is a group of more than 150 related viruses. Cervical cancer screening is usually part of a woman's health checkup. There are several types of tests: ThinPrep PAP test, the HPV test and Cintec Plus test. The aim of this study was to evaluate the diagnostic performance of the Cintec Plus immunocytochemistry test in detecting precancerous cervical disease compared to HPV testing and more traditional screening methods like Pap cytology. The objectives: 1. Assess the relationship between cervical lesions and woman's age. 2. To assess the relationship between the HPV DNA test results and the specific type of HPV and the age of women. 3. Determine the relationship between the cytology PAP test outcome and the prevalence of HPV infection. 4. Determine the relationship between the cytology PAP test results and the Cintec Plus test results. 5. To evaluate the relationship between HPV DNA and Cintec Plus test results for cervical pathology. Methods. A total of 192 samples were collected from women who attending routine cervical cancer screening between April 2015 and November 2015. ThinPrep PAP cytology, p16/Ki-67 immunostaining, and cobas HPV DNA testing were performed on all women. HPV-DNA detection was performed by cobas HPVtest for 14 high oncogenic risk HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 DNA in the clinical material (cervical swabs). Results There were no statistically significant differences in age between patients diagnosed with dysplasia. The association between the age range and the HPV positive result of the HPV DNA test in women's age was found among the age group (40-44) and the highest age group with a positive HPV DNA test (30 -34). A statistically significant relationship was found between cytological diagnosis (LSIL, HSIL and IPPN) and HPV DNA test results (p<0,001). A statistically significant relationship was found between the cytological diagnosis and immunocytochemical results of Cintec Plus (p <0.001). Keyword. ThinPrep PAP smear, HPV, Cintec Plus test; cervical cancer. |
