| Abstract [eng] |
Carbapenems are β-lactam antibiotics commonly used for treatment of Enterobacteriaceae infections. Enterobacteriaceae (Gram-negative bacili) are inhabitants of the intestinal flora and are the common human pathogens causing infections (Nordmann et al., 2011). The prevalence of carbapenem-resistant Enterobacteriaceae is increasing (Li et al., 2018). Infections caused by these bacterias have been associated with high mortality rates of approximately 40 % (Tangden ir Giske, 2015). One of the carbapenem resistance mechanisms is production of the carbapenemase or other β-lactamase (Walsh, 2010). The purpose of this work was to determine if the carbapenem resistant or less sensible Enterobacteria extracted in the VUH SC Microbiology laboratory contain KPC, NDM, VIM, OXA-48, IMP, GES karbapenemase and ACT, CMY-2, DHA AmpC β-lactamase genes. For this purpose we used RT-PCR which are suggested as the gold standard technique for identification of carbapenemase or AmpC β-lactamase genes (Smiljanic et al., 2017; Helmy ir Wasfi, 2014). According to the results, the prevalence of GES and OXA-48 carbapenemase genes reaches 1 % and 0,5 %, whereas GIM, VIM, NDM, IMP carbapenemase genes haven`t been detected. AmpC β-lactamase genes were more prevalent. The prevalence of ACT, CMY-2 and DHA genes reaches 10,7 %, 2,1 % and 0,5 %. According to the results obtained, production of carbapenemases and AmpC β-lactamases isn`t the only reason why Enterobacteria extracted in the VUH SC Microbiology laboratory have resistance to carbapenems. |
