| Abstract [eng] |
The Effect of Oxidative Stress on Platelet Membrane Fatty Acids Composition The aim of the study was to evaluate the effect of oxidative stress on platelet memebrane fatty acid composition. Introduction. Oxidative stress causes lipid peroxidation and the peroxidation process in the platelet membrane provides a variety of lipid peroxides including malondialdehyde, which has been implicated in the ethiology of cardiovascular diseases. Some polyunsaturated fatty acids (PUFAs) of platelet membrane are the major substrate for the biosynthesis of eicosanoids, which have well-established roles in regulation of inflammation, immunity and platelet activation. Materials and methods. Platelets membrane fatty acids methyl esters of 79 healthy men (aged 36,5±10,8; and without any acute clinical condition at the time) were identified by gass chromatography – mass spectrometry, while malondialdehyde was measured by high performance liquid chromatography in blood serum. Individuals were divided into quartiles according to the blood serum malondialdehyde concentration. The concentration of blood serum malondialdehyde was compared to the composition of platelets membrane fatty acids of apparently healthy individuals. Results. The total sum in percentage of saturated fatty acids compared to the lowest and the highest blood serum malondialdehyde concentration was decreased, while the level of polyunsaturated and monounsaturated fatty acids increased. No statistically significant differences in platelets membrane fatty acids composition between the highest and the lowest concentration of blood serum malondialdehyde, except 22:5ω3, which was significantly higher in the higest blood serum malondialdehyde concentration (p=0,038) and 18:2ω6/20:4ω6 ratio, which was significantly higher in the lowest blood serum malondialdehyde concentration (p=0,038). Blood serum malondialdehyde concentration of healthy individuals has statistically significant correlation of 14:0 fatty acid (r=-0,255; p=0,023) and 18:2ω6/20:4ω6 ratio (r=-0,244; p=0,034). Conclusion. Malondialdehyde concentration is a factor which stimulates platelets to synthesize PUFAs and eicosanoids/docosanoids which are required for platelet activation. Keywords. Oxidative stress, peroxidation, malondialdehyde, platelets membrane, fatty acids. |
