| Abstract [eng] |
Master’s degree thesis: Prevalence of clinically relevant non-tuberculous mycobacterium and antimicrobial resistance detection at Vilnius University Hospital Santaros Klinikos in 2015 – 2018. Aim of the study: evaluate the prevalence of clinically relevant nontuberculous mycobacteria, species diversity and to investigate their resistance to antibacterial drugs at Vilnius University Hospital Santaros Klinikos during 2015-2018 period. Materials and methods. The study was conducted at Vilnius University Hospital Santaros Klinikos Center of Laboratory Medicine Tuberculosis Laboratory. A retrospective analysis of 166 nontuberculous mycobacteria was performed. Macrolides and aminoglycosides resistance for M. avium complex, M. abscessus complex and M. chelonae was determined by using GenoType® NTM-DR VER 1.0 commercial kit, a total of 51 cultures were tested. Results. During the retrospective analysis, the most isolated nontuberculous mycobacteria were M. avium – 51.2% (N = 85), M. gordonae - 19.9% (N = 33) and M. fortuitum 7.2% (N = 12), and less commonly found were M. abscessus complex of 1.8% (N = 3), M. chelonae 4.2% (N = 7), M. kansasii 3% (N = 5), M. xenopi 2.4% (N = 4), M. intracellulare 1.8% (N = 3), M. celatum 1.8% (N = 3). None of the investigated nontuberculous mycobacteria had mutations in rrl and rrs genes, indicating macrolides and aminoglycoside resistance respectively. However, a T nucleotide was determined at 28 position of erm(41) gene of M. abscessus subsp. abscessus and M. abscessus subsp. bolletii, associated with resistance to macrolides. Conclusions. The most frequently isolated nontuberculous mycobacteria at Vilnius University Hospital Santaros Klinikos were M. avium, M. gordonae and M. fortuitum during 2015 – 2018 year period. None of the analyzed nontuberculous mycobacteria exhibited mutations showing acquired resistance to macrolides and aminoglycosides. However, an erm(41) gene polymorphism was identified for two isolates - M. abscessus subsp. abscessus and M. abscessus subsp. bolletii showing resistance to macrolides. Detection of common gene mutations of nontuberculous mycobacteria, associated with resistance to macrolides and aminoglycosides, using PCR method is reasonably good, although results should be interpreted in consideration with other clinical tests. |
