| Abstract [eng] |
The Assessment of Blood Serum Total Antioxidant Activity of Males Suffering from Different Levels of Stress Introduction. Stress is an organism's response to a stressor, during exposure of stress endogenous protective mechanisms existing in living cells may become insufficient and cause an imbalance between pro-oxidative factors and antioxidants that leads to increased oxidation. Aim. The aim of this work was to evaluate total antioxidant activity in blood serum of men suffering from different levels of stress. Materials and methods. 220 healthy males aged 25-54 years were subjects of this research. Stress level was evaluated by the concentration of cortisol in hair: individuals with cortisol concentrations below the median (<45,6250ng/g) – classified as having low level of stress, individuals with cortisol concentration above the median were classified as having high level of stress. Total antioxidant activity (TAA) was determined by the ferric reducing ability of plasma (FRAP) assay and Tween 80 oxidation test (the data was expressed as the percentage of reduction rate). Microsoft Office Excell 2010 and R version 3.4.3 software packages were used for statistical analysis. Results. Median of TAA assessed using FRAP assay was 47.68 μM, using Tween 80 – 43.37%. In the group of study individuals with low level of stress TAA was 46.20 μM and 43.78% respectively by FRAP and Tween 80.In the group of study individuals with high level of stress TAA was 49.14 μM and 42.63 % respectively by FRAP and Tween 80. FRAP assay has shown statistically significantly higher TAA in the group of study individuals with low level of stress (r=0.3054, p=0.0062), but not a Tween 80 test (r= -0.1587, p = 0.1653). Conclusions. FRAP assay but not a Tween 80 oxidation test has shown the difference of total antioxidant activity between different levels of stress evaluated by the concentration of cortisol in hair. |
