| Abstract [eng] |
Ardano Kozyro master’s thesis: Auricularia auricula-judae biological activity assays. Aim of study: To assess pharmacological activity of Auricularia auricula-judae fruitbody extracts. Objectives of study: 1) To determine the total phenolic content of compounds and antioxidant activity of Auriculariaauricula-judae fruitbody extracts; 2) To qualifiedly evaluate non-volatile compounds in wood ear fruitbody extracts using liquid chromatography method; 3) To identify volatile compounds in wood ear fruitbody extracts using gass chromatography method; 4) To reveal toxic activity of the extracts against Artemia sera larvae. Methods: Fruitbodies of wood ear was gathered, dried and pulverised. Bioactive compounds were extracted byusing different polarity solvents: water, methanol, dichloromethane and hexane. Total phenolic compound was determined by using calorimetric Folin-Ciocalteu method, expressed in mg GAE/g(galic acid equivalents) of DW (dry weight). Radical scavenging activity was evaluated by usings pectrophotometric DPPH· and ABTS·+ assays, expressed in μM TE/g (trolox equivalents) DW. Volatile compounds were identified using GS/MS, while phenolic constituents were tentatively identified by HPLC/DAD/TOF. Toxicity of A. auricula extract were evaluated using Artemia seranauplius as a model animal. Results and concusions: Total phenolic content of compounds was found to be the highest in aqueous extract (3,23± 0,18 mgGRE /g DW) and dwindled with decreasing ratio of water in a mixture (1,78± 0,04 and 0,92± 0,12mg/g DW). Antioxidant activity was highest in water extract diluted 1:1 with 80% H₂O:MeOH mixture and filtered through syringe (4,72 ± 0,2 μM TE/ g and 9,39 ± 0,67 μM TE/ g DW). Followedby unprocessed water extract 2,09 ± 0,5 μM and 6,84 μM ±0,55 TE/ g DW for DPPH and ABTSradical scavenging assays, respectively. Similar tendency was observed by comparing precipitatedand non-precipitated extracts, where the centrifuged one showed ~40% higher activity. This could beexplained by elimination of radical shielding compounds from solution. Antioxidative activity could be linked to vanillic, ascorbic, chlorogenic acid derivatives, flavonoid 5,7-dihydroxychromone aswell as melanin identified in HPLC/DAD/TOF. Fatty acids esters, sesquiterpene β-selinene, branchedphenolic acid and long-chain alkanes identified by GC/MS could contribute to anti-inflammatory,7anti-bacterial activities and gut microbiota modulation. All bioactive activities listed above wereachieved without any acute toxicity for Artemia sera nauplius. |
