| Abstract [eng] |
In plants, electrical signals - action potentials (APs) - are essential for long-distance information transmission and coordinated responses to environmental stress. While AP elicitation and transmission to neighboring cells are crucial for systemic responses, positive correlates of AP transmission likelihood are largely unknown. The study aimed to characterize the electrophysiological properties of Nitellopsis obtusa’s node in a system of tandem and investigate its alterations under exposure to Glu. The objectives of the study were to (1) To characterize the electrophysiological properties of the node under control conditions (2) To examine whether AP transmission from one internode to the adjacent internode is associated with the electrophysiological properties of the node (3) investigate the effect of 1 mM Glu exposure on electrophysiological properties of the node. Using a two intracellular electrode setup, the transnodal potential of Nitellopsis obtusa’s tandem was recorded and nodal resistance was calculated from the applied current using Ohm’s law. APs were elicited using cold stimulation and AP transmission probability was estimated in control and Glu conditions. Results indicate that transnodal resistance and transnodal potential remain stable in the absence of electrical excitation. In contrast, with repeated cell excitation, transnodal resistance increases depending on the number of APs. Meanwhile, transnodal potential, and action potential transmission probability between the cells remain unaffected. AP transmission probability between internodal cells was not correlated with the transnodal resistance in control conditions. Tandem exposure to 1 mM Glu significantly increases transnodal resistance, while marginally decreasing action potential transmission probability. |
