| Title |
The development and evaluation of Schmallenberg virus detection systems / |
| Translation of Title |
Antikūnų prieš Schmallenberg virusą detekcijos sistemos sukūrimas ir vertinimas. |
| Authors |
Lazutka, Justas |
| Full Text |
|
| Pages |
176 |
| Keywords [eng] |
Schmallenberg virus ; immunoassay ; cattle ; recombinant proteins ; saliva |
| Abstract [eng] |
Schmallenberg virus (SBV) is an arthropod-borne virus. It infects cattle and causes severe malformations in their offspring. The aim of this study was to develop an enzyme-linked immunosorbent assay by using recombinant SBV structural proteins synthesized in S. cerevisiae. The developed system could provide an alternative cheaper tool for scientists to evaluate the state of SBV infection in cows. SBV N and SBV N-His proteins were purified from S. cerevisiae and used to create immunoassays that detect antibodies against SBV. Bovine serum specimens collected in Lithuanian and Ukrainian farms were evaluated using commercial and newly developed assays. The rate of infection was 87% in 2013 and 12%–17% in 2014. The sensitivities of the newly developed tests in comparison with the commercial tests were as follows: 95% for the IgG serum assay, 94.12% for the IgG milk assay, 84.91% and 98.15% for IgG and IgA in saliva tests. The MAbs raised against recombinant SBV N protein reacted with native viral nucleocapsids in SBV-infected mammalian cells. This shows the antigenic similarity between recombinant and native viral nucleocapsids. Data from testing the saliva IgG and IgA and milk and serum IgG with indirect SBV-specific ELISAs showed close agreement with the commercial serum and milk IgG assay data. This work also shows for the first time that the bovine saliva samples can be used for SBV/Simbu serogroup virus diagnostics. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Doctoral thesis |
| Language |
English |
| Publication date |
2018 |
