| Abstract [eng] |
In this research, we have discovered that L A virus is widespread among the most popular laboratory strains of S. cerevisiae yeast used in scientific research. The presence of L A virus is not associated with changes in cell viability or growth rate, however the data obtained in recent years show that the presence of the virus in the cell changes a large part of the host's gene expression. For this reason, transcriptomic or proteomic analysis data may be misinterpreted in the presence of a virus, because observed changes in gene expression may not necessarily be related to the studied process, but also may be related to the change in the amount and existence of the virus in the cell. In this work we have found new, unpublished host cell proteins interacting with proteins encoded by L-A virus. We have identified new L A virus and M satellite genomes from S. paradoxus and S. cerevisiae killer strains. We have found the direct relationship between the phylogenetic groups formed by different variants of the L A virus and the host species. Additionally, distribution in phylogenetic groups is based on strain origin and ability to provide a specific M satellite replication. |
