| Title |
A selective phenyl azo acetate substrate for enhanced carbonic anhydrase functional analysis |
| Authors |
Babinskas, Justinas ; Matijošytė, Inga |
| DOI |
10.1002/ansa.70091 |
| Full Text |
|
| Is Part of |
Analytical science advances.. Wiley-VCH GmbH.. 2026, vol. 7, iss. 1, art. no. e70091, p. [1-13].. eISSN 2628-5452 |
| Abstract [eng] |
Carbon dioxide (CO2) sequestration is a key strategy for mitigating climate change, and biocatalytic approaches offer sustainable, cost-effective alternatives to chemical and physical methods. Carbonic anhydrases (CAs) efficiently catalyse the reversible hydration of CO2 to bicarbonate, yet their large-scale application is constrained by limited stability and the lack of robust analytical tools for activity and/or inhibition profiling. In this work, we synthesised and characterised a novel phenyl azo dye acetate to address these limitations. The substrate was optimised for spectrophotometric assays, enabling reliable kinetic and inhibition analyses of CAs. Compared with conventional substrates such as p-nitrophenyl acetate and indoxyl acetate, the new assay demonstrated more than 4.5-fold higher sensitivity, substantial thermal stability and wider applicability across tested hydrolases. These findings provide a more robust platform for evaluating CA activity, supporting the development of more efficient biocatalytic systems for CO2 sequestration. |
| Published |
Wiley-VCH GmbH |
| Type |
Journal article |
| Language |
English |
| Publication date |
2026 |
| CC license |
|
