| Title |
Patologinių nervų sistemos audinių cheminis vaizdinimas naudojant tiesinę ir netiesinę virpesinę mikroskopiją / |
| Translation of Title |
Linear and non-linear vibrational microscopy-based chemical imaging of pathologic central nervous system tissue. |
| Authors |
Tamošaitytė, Sandra |
| Full Text |
|
| Pages |
62 |
| Keywords [eng] |
FT-IR microspectroscopy ; Raman microspectroscopy ; CARS microscopy ; multimodal non-linear optical microscopy ; central nervous system |
| Abstract [eng] |
Due to the growing need of improvements in central nervous disease assessment, the importance to establish sensitive, chemically selective and label-free imaging techniques is of a great demand. The main goal of this work was to combine experimental vibrational spectroscopy-based imaging techniques and spectral data analysis approaches to (i) evaluate these imaging modalities as a tool for diagnostic neuropathology and monitoring of spinal cord injury treatment strategies and to (ii) gain new insights in biochemical status of diseased nervous tissue. The effects of brain tissue sections deparaffinization was analyzed in order to evaluate compatibility of paraffinized brain tissue samples for multimodal coherent anti-Stokes Raman scattering (CARS) imaging. Also, CARS microscopy was validated as a tool for precise delineation and assessment of primary and secondary brain tumors as well as biopsies of human brain tumors. These results were confirmed by Raman microspectroscopy and histochemistry. Linear vibrational microspectroscopy and multimodal CARS microscopy were applied for assessing the effects of spinal cord injury treatment. Various chemometrical spectral data analysis approaches, which enabled to assess the nervous tissue demyelination, scarring and lipid changes associated with inflammation triggered by spinal cord injury, are presented. |
| Dissertation Institution |
Vilniaus universitetas. |
| Type |
Summaries of doctoral thesis |
| Language |
Lithuanian |
| Publication date |
2018 |
