| Title |
DNA extraction optimisation for minute land snails of vertigo Müller, 1773 (Gastropoda: vertiginidae): a comparative evaluation of six methods, including a non‐destructive shell‐preserving protocol |
| Authors |
Jukonis, Benediktas ; Havelka, Jekaterina ; Skujienė, Grita |
| DOI |
10.1002/ece3.74295 |
| Full Text |
|
| Is Part of |
Ecology and evolution.. Hoboken : John Wiley and Sons Ltd. 2026, vol. 16, iss. 9, art. no e74295, p. [1-16].. eISSN 2045-7758 |
| Abstract [eng] |
No systematic comparison of DNA extraction strategies exists for minute Vertiginidae (shell height 51). The shell‐preserving non‐destructive HotSHOT protocol yields sequenceable DNA from protected Vertiginidae while retaining the morphological voucher, making it the preferred option for conservation‐genetic monitoring. The practical decision framework documented here—integrating voucher preservation, amplification robustness and per‐sample cost—has broad applicability to other minute terrestrial gastropods processed in large‐scale biodiversity surveys. |
| Published |
Hoboken : John Wiley and Sons Ltd |
| Type |
Journal article |
| Language |
English |
| Publication date |
2026 |
| CC license |
|
