| Abstract [eng] |
The aim of the study was to evaluate the relationship between human papillomavirus (HPV) infection and gene TP53, MDM2, MDM4, MTHFR, CASP8 and CCR5 single nucleotide polymorphisms (SNPs) frequencies and clinical-pathological parameters, and their relevance to the survival of patients with laryngeal and lung cancers. The patient's cancer data (tumor stage, degree of differentiation, TNM, disease progression and patient survival rates) and lifestyle data were collected and analyzed during the study. At the begining the clinical-pathological data were evaluated for patients with laryngeal and lung cancer, and then patients were divided into groups based on a clinical-pathological data obtained from medical records. All collected biological samples were tested for HPV infection. Following, SNPs of selected genes were determined by PCR, RT-PCR and RFLP. Subsequently, all parameters were evaluated and overall analysis was performed in order to determine the interrelationships between the parameters and the importance of patient‘s survival indicators and the prognosis of the course of the disease. By analysing the interactions of the SNPs of the genes with clinical parameters, the majority of laryngeal cancer patients with lymphnode status N2 were identified as carriers of MDM2 T/G, CASP8ins/del, CCR5 wt/wt SNP. Cluster analysis showed that patients with MDM2 T/T SNP survive longer than patients identified as CASP8 ins/ins MTHFR C/C and MDM4 A/A variant carriers, meanwhile LSCC patients with MDM2 T/T polymorphic variant had the best survival. Multivariate analysis showed that HPV positive patients without metastasis in regional lymph nodes (N0) and harbored CASP8 ins/del variant had the best survival, hereby HPV negative patients with identified metastasis in lymph nodes (N1 and N2) and CASP8 ins/del variant had poor survival. Interestingly, we determined statistically significant link between the poor survival of lung cancer patients after surgery and the combination of polymorphic variants C/T of the MTHFR gene and T/T of the MDM2 gene, wheareas individually these SNPs do not show significant relationship with the survival of patients after surgery. |
