| Title |
Degradation of naphthalene by thermophilic bacteria via a pathway, through protocatechuic acid |
| Authors |
Bubinas, Audrius ; Giedraitytė, Gražina ; Kalėdienė, Lilija ; Nivinskienė, Ona ; Butkienė, Rita |
| DOI |
10.2478/s11535-007-0042-X |
| Full Text |
|
| Is Part of |
Central European journal of biology.. Warsaw : Versita. 2008, vol. 3, no. 1, p. 61-68.. ISSN 1895-104X |
| Keywords [eng] |
Bacillus-thermoleovorans ; Strain ; Phenanthrene ; Metabolism ; Biodegradation ; Transformation ; 1-naphthol ; Phenol ; Carbon |
| Abstract [eng] |
A number of thermophilic bacteria capable of utilizing naphthalene as a sole source of carbon were isolated from a high-temperature oilfield in Lithuania. These isolates were able to utilize several other aromatic compounds, such as anthracene, benzene, phenol, benzene-1, 3-diol, protocatechuic acid as well. Thermophilic isolate G27 ascribed to Geobacillus genus was found to have a high aromatic compound degrading capacity. Spectrophotometric determination of enzyme activities in cell-free extracts revealed that the last aromatic ring fission enzyme in naphthalene biotransformation by Geobacillus sp. G27 was inducible via protocatechuate 3, 4-dioxygenase; no protocatechuate 4, 5-dioxygenase, protocatechuate 2, 3-dioxygenase activities were detected. Intermediates such as o-phthalic and protocatechuic acids detected in culture supernatant confirmed that the metabolism of naphthalene by Geobacillus sp. G27 can proceed through protocatechuic acid via ortho-cleavage pathway and thus differs from the pathways known for mesophilic bacteria. |
| Published |
Warsaw : Versita |
| Type |
Journal article |
| Language |
English |
| Publication date |
2008 |
